APPL1 Antibody MA5-26917 from Thermo Fisher Scientific

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WB

Western blot was performed using Anti-APPL1 Mouse Monoclonal Antibody (Product # MA5-26917) and 79 kDa band corresponding to APPL1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), SW480 (Lane 2), MCF7 (Lane 3), HeLa (Lane 4), HEK-293 (Lane 5), Mouse Brain (Lane 6), Rat Brain (Lane 7), Mouse Spleen (Lane 8) and Mouse Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A 25 kDa band (*) corresponding circulating tissue IgG was observed in mouse tissues.
ICC/IF

Immunofluorescence analysis of APPL1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with APPL1 Monoclonal Antibody (Product # MA5-26917) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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Thermo Fisher Scientific for
APPL1 Antibody

Thermo Fisher Scientific's APPL1 Antibody is a Mouse monoclonal antibody. The APPL1 Antibody was generated using appl1 as the antigen. It reacts with Homo Sapiens, Human, Mouse, and Rat. This antibody has been shown to work in applications such as: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.

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ICC/IF

APPL1 Antibody from Thermo Fisher Scientific

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APPL1 Antibody from Thermo Fisher Scientific

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